Export Formats

qPCR CopyCount requires that you upload your raw qPCR fluorescence data for each cycle as a text based CSV/TSV file or Excel XLS/XLSX formats. At this time we are unable to support direct binary formats such as SDS and EDS files. Currently, we can automatically detect the following formats:

  • Abbott m2000rt
  • ABI 7500/7900
  • Bio-Rad:  CFX and iCycler
  • Life Technologies: ViiA 7, Step One, OpenArray 12K Flex
  • Qiagen: Rotor-Gene
  • Roche: LC480 and Lightcycler Series
  • StrataGene MX
  • General Column-Layout CSV
  • General Row-Layout CSV

If your data is not in one of the above formats, please contact DNA Software and we will work on adding it to the system!

Export Instructions

ABI 7500

  1. Open the project file.
  2. Click the Export in the tool bar
  3. Go to File / Export / Right click on export spectra
  4. Ensure only the Sample Setup and Raw Data checkboxes are checked.
  5. Select One File from the drop down.
  6. Enter the Export FIle Name and ensure that the File Type is set to (*.txt)
  7. Set the Export File Location
  8. Click Start Export to export your raw fluoroescence data. You may now upload this file to qPCR CopyCount

ABI 7900

  1. Click on the plate document to select it.
  2. In the File menu, select Export
  3. Navigate to the directory you would like to export the data files to.
  4. In the Export drop down list, select “Clipped”
  5. Select All Wells, you will have the opportunity to narrow the selction down in the qPCR CopyCount tool.
  6. In the File name box, enter the name ofr the exported data set.
  7. Click Export. You may now upload this file to qPCR CopyCount

LightCycler

  1. Browse to your experiment in the Navigator.
  2. Select the Export function in the LightCycler Navigator.
  3. Select the experiment that you would like to export, click “Export” at the bottom of the screen.
  4. Select “Experiment Text File” as the file type you would like to export.
  5. Click Export. You may now upload this file to qPCR CopyCount

Bio-Rad CFX

  1. Open your experiment results.
  2. Click Export
  3. Choose “Export All Datasheets to Excel”
  4. Choose a save location. We recommend using an empty folder due to the large number of files exported.
  5. When uploading to qPCR CopyCount, choose the file ending with “- Quantification Amplification Results”

StrataGene MX

  1. Open your experiment results.
  2. Click File and then click Export Instrument Data
  3. Choose “Export Instrument Data to Excel”
  4. Choose “Format 1”
  5. Choose a save location. You may now upload this file to qPCR CopyCount

Abbott m2000rt

  1. Select Results, then View By Plate
  2. Select the desired PCR plate
  3. From the Plate Tasks menu, select Export
  4. Go to the section of the screen that says Exporting Plate Results and then to the section Export File Names.  The .csv file contains the raw fluorescence data that is needed for qPCR CopyCount.
  5. Save the .csv file to the computer hard drive, CD, or flash drive.  Be sure to give the file an informative name so you know what data is in that file.  Note the folder location of that file since you will need to browse to that location when using qPCR CopyCount.

Step One

  1. Click on Export under the file menu bar
  2. Deselect results and check raw data in the top section
  3. Click Start Export on the bottom right
  4. You may now upload this file to qPCR CopyCount

ViiA 7

  1. Select Export button on lower left side of software
  2. Deselect all tabs except for the Raw Data Tab
  3. Choose the data file for export and select Start Export
  4. You may now upload this file to qPCR CopyCount