Visual OMP  is also able to design allele specific primers and probes over or around single SNP sites for the binary IUPAC ambiguity codes. In this example a C. albicans sequence with a W ambiguity code (A or T) is 97.95 % differentiated by Visual OMP using two allele specific forward primers and a common reverse primer. Multiple SNP sites or ambiguity codes are not supported at this time.

nucleotide