Large Scale Multiplex PCR Panel Design
Large Scale Multiplex PCR Panel Design with >95% Success in Hours, Not Months
John SantaLucia, Ph.D., co-founder and CEO, DNA Software
Abstract: We aim to solve the problem of multiplex PCR design by a three-pronged approach: 1. improved understanding of the mechanism of PCR and the causes of artifacts, 2. improved algorithms for predicting secondary structure and mis-hybridization, and 3. implementation of cloud to address large sequence databases. These approaches vastly improve the success of fully automated multiplex PCR design with larger plex sizes. This enables applications such as targeted enrichment for NGS and infectious disease molecular diagnostics.