A. Within DNA the following 5-letter codes are supported:
DNA_I = deoxyInosine
DNA_U = deoxyUridine
DisoC = isoC with deoxy sugar
DisoG = isoG with deoxy sugar
D_DAP = DiAminoPurine in DNA
D_gcC = G-clamp C in DNA
D_2fC = 2′ fluoroC in DNA
D_2fU = 2′ fluoroU in DNA
D_5mC = 5-methyl-C in DNA
D_5pC = 5-propynyl-C in DNA
D_5pU = 5-propynyl-U in DNA
D_2tT = 2-thio Thymine in DNA
D_2ap = 2-amino purine in DNA
DNA_Z = 7-deazaG in DNA
OM2_A = 2′-O-methyl adenosine
OM2_C = 2′-O-methyl cytidine
OM2_G = 2′-O-methyl guanosine
OM2_U = 2′-O-methyl uridine
LNA_A = LNA adenosine
LNA_C = LNA cytidine
LNA_G = LNA guanosine
LNA_T = LNA Thymidine
LNA_U = LNA uridine
RNA_A = RNA adenosine chimeric in DNA
RNA_C = RNA cytidine chimeric in DNA
RNA_G = RNA guanosine chimeric in DNA
RNA_U =RNA uridine chimeric in DNA
In our next release we will also be supporting:
D_2fA = 2′ fluoroA in DNA
D_2fG = 2′ fluoroG in DNA
D_2fT = 2′ fluoroT in DNA
B. Within RNA the following 5-letter codes are supported:
RNA_T = RNA backbone with Thymidine base
OM2_A = 2′-O-methyl adenosine
OM2_C = 2′-O-methyl cytidine
OM2_G = 2′-O-methyl guanosine
OM2_U = 2′-O-methyl uridine
DNA_A = DNA adenosine chimeric in RNA
DNA_C = DNA cytidine chimeric in RNA
DNA_G = DNA guanosine chimeric in RNA
DNA_T =DNA thymidine chimeric in RNA
LNA_A = LNA adenosine
LNA_C = LNA cytidine
LNA_G = LNA guanosine
LNA_T = LNA Thymidine
LNA_U = LNA uridine
D_2fC = 2′ fluoroC with DNA backbone
D_2fU = 2′ fluoroU with DNA backbone
D_5pC = 5-propynyl-C with DNA backbone
D_5pU = 5-propynyl-U with DNA backbone
RisoC = isoC in RNA
RisoG = isoG in RNA
R_DAP = DiAminoPurine in RNA
R_5mC = 5-methyl-C in RNA
RNA_Z = 7-deazaG in RNA
RNA in the future will support:
PSD_A = Phosphorothioate A with DNA sugar chimeric in RNA
PSD_C = Phosphorothioate C with DNA sugar chimeric in RNA
PSD_G = Phosphorothioate G with DNA sugar chimeric in RNA
PSD_T = Phosphorothioate T with DNA sugar chimeric in RNA
C. Within PNA the following 5-letter codes are supported:
PNA_Z = 7-deazaG in PNA
P_DAP = diaminopurine in PNA
PNA Fluorophores and quenchers: 5’-Texas Red, 5’-Cy3, 3’-BHQ, 3’-TAMRA
D. Within MOR the following 5-letter codes are supported:
No modifieds allowed
Fluorophores allowed: 3’-Lissamine, 3’-FAM, 3’-Dabcyl, 3’-Biotin.
E. For DNA and RNA here are the 3′-quenchers and 5′-Fluorophores that
we support. There is also support for terminal phosphate, biotin,
certain PEG linkers. We also have some support for internal
fluorophores (inquire if interested). Here is the complete llist of
Fluorophores and quenchers: