Antisense Webinar Transcript
Better Antisense Design Without the Trial and Error: Problems we Solve
Most researchers use naïve strategies to design antisense oligonucleotides. However, to maximize assay sensitivity and specificity one must consider probe and target sequence and their respective and combined chemistries. Read more ›
The Real Cost of Multiplex
We recently spoke with a customer who was sharing with us the trials and tribulations of developing a multiplex PCR assay with multiple experimental iterations, at least a half dozen. When we starting asking clarifying questions, and we were surprised Read more ›
DNA Software partners with Insilixa to provide high multiplex infectious disease detection panels
InSilixa Inc. and DNA Software Inc. enter into agreement to develop state-of-the-art high-multiplex infectious disease panels for commercialization on InSilixa’s CMOS biochip platform
03/10/2016 See press coverage at Genome Web
Infectious disease molecular diagnostic (MDx) assays developed specifically for InSilixa’s Read more ›
How do you design a successful multiplex PCR?
How do you design a successful multiplex PCR?
This question has been a common theme among DNAS customers since our inception 15 years ago. Whether the multiplex is used as a molecular diagnostic or for gene enrichment libraries for NGS,
Read more ›Myth 7: Multiplex PCR Can Succeed by Optimization of Individual PCRs
Not too many people believe this myth, and yet their actions are somewhat irrational as they proceed to immediately use that approach to try to experimentally optimize a multiplex PCR. It is true that well-designed single-target PCRs are useful for
Myth 6: At the End of PCR, Amplification Efficiency Is Not Exponential Because the Primers or NTPs Are Exhausted or the Polymerase Looses Activity
PCR amplification occurs with a characteristic “S” shape. During the early cycles of PCR, the amplification is exponential. During the later stages of PCR, saturation behavior is observed, and the efficiency of PCR decreases with each successive cycle. What is
Myth 5: A BLAST Search Is the Best Method for Determining the Specificity of a Primer
To minimize mispriming, several PCR texts suggest performing a BLAST search, and such capability is a part of some primer design packages such as GCG and Vector NTI and Visual-OMP. However, a BLAST search is not the appropriate screen for
Myth 4: “Primer Dimer” Artifacts Are Due to Dimerization of Primers
A common artifact in PCR is the amplification of “primer dimers.” The most common conception of the origin of primer dimers is that two primers hybridize at their 3′-ends (see Fig. 8). DNA polymerase can bind to such species and
Myth 3: Designing Forward and Reverse Primers to Have Matching Tm’s Is the Best Strategy to Optimize for PCR
Nearly all “experts” in PCR design would claim to believe in myth 3. Most current software packages base their design strategy on this myth. Some careful thought, however, quickly reveals the deficiencies of that approach. The Tm is the temperature