Polymorphism Design and Simulation

Visual OMP  is also able to design allele specific primers and probes over or around single SNP sites for the binary IUPAC ambiguity codes.

In this example a C. albicans sequence with a W ambiguity code (A or T) is 97.95 % differentiated by Visual OMP using two allele specific forward primers and a common reverse primer.

Multiple SNP sites or ambiguity codes are not supported at this time.

nucleotide