Myth 7: Multiplex PCR Can Succeed by Optimization of Individual PCRs
Not too many people believe this myth, and yet their actions are somewhat irrational as they proceed to immediately use that approach to try to experimentally optimize a multiplex
Not too many people believe this myth, and yet their actions are somewhat irrational as they proceed to immediately use that approach to try to experimentally optimize a multiplex
To minimize mispriming, several PCR texts suggest performing a BLAST search, and such capability is a part of some primer design packages such
A common artifact in PCR is the amplification of “primer dimers.” The most common conception of the origin of primer dimers is that two primers hybridize at their 3′-ends
Nearly all “experts” in PCR design would claim to believe in myth 3. Most current software packages base their design strategy
Best methods for predicting hybridization Tm are essentially equivalent in accuracy. The melting temperature, Tm, of duplex formation is usually defined as the temperature at which half the available strands are in
It might come as a surprise to many that despite the wide use and large investment, PCR in fact is still subject to many artifacts and environmental factors Read more ›
Difference between traditional quantitative pcr with computer-based quantitation algorithm for cmv. Traditional viral quantitation using cycle threshold (Ct) analysis is dependent on generating an
DNA Software introduced the full commercial release of ThermoBLAST Cloud Edition (TB-CE). TB-CE provides a new standard for evaluating the target specificity of oligonucleotides.
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