Fluorophores and Quenchers

Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescentchemical compound that can re-emit light upon light excitation

Quencher

Quenching refers to any process which decreases the fluorescence intensity of a given substance

3 Primer Quenchers:

  •  BHQ-1 (Black Hole Quencher 1)
  •  BHQ-2 (Black Hole Quencher 2)
  •  Dabcyl
  •  Iowa Black FQ 420-620 max 531
  •  Iowa Black RQ 500-700 max 656
  •  QSY 7 amine
  •  QSY 9 hydrazide
  •  QSY 35 methylamine

5′ Fluorophores:

  •  Alexa Fluor 350 cadaverine
  •  Alexa Fluor 430 cadaverine
  •  Alexa Fluor 488 cadaverine
  •  Alexa Fluor 532 cadaverine
  •  Alexa Fluor 546 cadaverine
  •  Alexa Fluor 568 cadaverine
  •  Alexa Fluor 594 cadaverine
  •  Alexa Fluor 633 cadaverine
  •  Alexa Fluor 660 cadaverine
  •  Alexa Fluor 680 cadaverine
  •  Bodipy 630/650-X NHS Ester
  •  Bodipy 650/665-X NHS Ester
  •  7-diethylaminocoumarin-3-carboxylic acid
  •  Cy-2 (Cyanine 2)
  •  Cy-3 (Cyanine 3)
  •  Cy-3.5 (Cyanine 3.5)
  •  Cy-5 (Cyanine 5)
  •  Cy-5.5 (Cyanine 5.5)
  •  Cycler 640
  •  DY547
  •  DY647
  •  DY677
  •  FAM (Fluorescein Amidite)
  •  HEX (Hexachlorofluorescein)
  •  VIC
  •  Dimethoxyfluorescein
  •  Lissamine
  •  MGB (Minor Groove Binder)
  •  Oregon Green 488-X NHS Ester
  •  Oregon Green 514 NHS Ester
  •  Pyrene
  •  Rhodamine Green NHS Ester
  •  Rhodamine Red NHS Ester
  •  6-carboxy-X-rhodamine
  •  Carboxytetramethylrhodamine
  •  TET (Tetrachlorofluorescein)
  •  Texas 615
  •  Texas Red cadaverine
  •  Texas Red-OO
  •  TYE 563
  •  TYE 665