The Problem With Absolute Quantification
Obtaining absolute PCR quantification currently requires the laborious preparation of standards and acquiring a standard curve, thereby wasting reagents and using valuable plate real estate. In addition, the traditional qPCR analysis method determines the “cycle threshold”, Ct or Cq, which varies for different assays, different machines, and varies from plate to plate, thereby making the Ct value hard to interpret.
Solving Absolute Quantification with qPCR CopyCount
DNA Software has made a breakthrough in understanding the mechanism of PCR amplification. Our new product, qPCR CopyCount™, allows for any qPCR curve to be analyzed to directly determine the absolute number of copies of DNA at cycle zero. The DNA copy count is the quantity that every biologist wants and the results provided by qPCR CopyCount™ have unprecedented relative and absolute accuracy.
How can qPCR CopyCount™ benefit your research as a Digital PCR alternative?
- DNA copy number results that are 3-4 X more precise than Ct method
- Eliminates two common sources of user error, namely the quantification of standards and the running of standard curves
- Accelerate your workflow with existing qPCR instrumentation
- Cost effective alternative to Digital PCR
Absolute Quantification with Existing qPCR Instruments
Utilizing your current lab equipment and methods, and providing you higher sample throughput than Digital PCR, qPCR CopyCount™ provides absolute quantification with outstanding accuracy, within 5% errors. Rather than the need for expensive new equipment, qPCR CopyCount™ makes every qPCR reaction an absolute reaction.
Lower Cost Per Well
qPCR CopyCount™ requires no quantification standards and provides users more sample wells rather than control wells. In other words, “more real estate for your plate”.
DNA Copy Number from qPCR Data
Obtain absolute quantification and DNA Copy Number of your qPCR data within 5% error.