News

Primer Dimer Artifacts Are Due to Dimerization

July 21, 2015

Myth 4: “Primer Dimer” Artifacts Are Due to Dimerization of Primers   A common artifact in PCR is the amplification of “primer dimers.” The most common conception of the origin of primer dimers is that two primers hybridize at their... Read more ›

Best Methods for Predicting Hybridization Tm

July 21, 2015

Myth 2: Best Methods for Predicting Hybridization Tm Best methods for predicting hybridization Tm are essentially equivalent in accuracy. The melting temperature, Tm, of duplex formation is usually defined as the temperature at which half the available strands are in... Read more ›

PCR Working and Design Is Not Important

July 21, 2015

Myth 1: PCR Nearly Always Works and Design Is Not that Important It might come as a surprise to many that despite the wide use and large investment, PCR in fact is still subject to many artifacts and environmental factors... Read more ›

Comparison of traditional quantitative pcr with computer quantitation

June 23, 2015

Comparison of traditional quantitative pcr with a computer-based quantitation algorithm for cmv from plasma specimens Background Difference between traditional quantitative pcr with computer-based quantitation algorithm for cmv. Traditional viral quantitation using cycle threshold (Ct) analysis is dependent on generating an... Read more ›

DNA Software releases ThermoBLAST Cloud Edition

November 7, 2014

DNA Software introduced the full commercial release of ThermoBLAST Cloud Edition (TB-CE). TB-CE provides a new standard for evaluating the target specificity of oligonucleotides. Click here to learn more

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