News
DNA Software partnership with Insilixa
March 10, 2016
InSilixa Inc. and DNA Software Inc. enter into agreement to develop state-of-the-art high-multiplex infectious disease panels for commercialization on InSilixa’s CMOS biochip platform See press coverage at Genome Web Infectious disease molecular diagnostic (MDx) assays developed in partnership with inSilixa... Read more ›
How do you design a successful multiplex PCR?
October 7, 2015
How do you design a successful multiplex PCR? This question has been a common theme among DNAS customers since our inception 15 years ago. Whether the multiplex is used as a molecular diagnostic or for gene enrichment libraries... Read more ›
DNA Software introduces the full commercial release of ThermoBLAST Cloud Edition (TB-CE).
July 25, 2015
ThermoBLAST Cloud Edition Overview ThermoBLAST Cloud Edition (TB-CE) provides a new standard for evaluating the target specificity of oligonucleotides during PCR primer or probe design. Everybody is familiar with the capabilities of NCBI BLAST, but there are no thermodynamic results and lots of... Read more ›
Counting PCR: A new method to obtain absolute DNA copy number without a standard curve
July 21, 2015
John SantaLucia, Jr. and Gregory J. Boggy DNA Software, Inc., Ann Arbor MI 48104 Abstract DNA Software has discovered how to analyze the shape of a PCR curve to reveal the absolute copy number of DNA at cycle zero. This... Read more ›
Quick Guide to the Precision and Accuracy of results from qPCR CopyCount
July 21, 2015
Purpose: This Quick Guide to the Output provides you with the basic description of QRT PCR data analysis and how to interpret the output results from qPCR CopyCount. Summary: An understanding of the difference between precision and accuracy is critical to... Read more ›
Two-step Assay Calibration Procedure for TaqMan Assays
July 21, 2015
Introduction The following procedure is performed on each new qPCR assay that will be analyzed by qPCR CopyCount. The method is called “2-step” because it involves two qPCR reactions: one preliminary PCR with 4 replicates to get a rough concentration,... Read more ›
Quick Start Guide for qPCR CopyCount
July 21, 2015
Purpose: This CopyCount Quick Start Guide provides the basic information and best practices for running qPCR CopyCount. Best practices for setting up your qPCR plate We recommend that each sample be run with at least 4 replicates. This allows for outliers... Read more ›
Multiplex PCR Optimization
July 21, 2015
Myth 7: Multiplex PCR Can Succeed by Optimization of Individual PCRs Not too many people believe this myth, and yet their actions are somewhat irrational as they proceed to immediately use that approach to try to experimentally optimize a... Read more ›
Amplification Efficiency for primers Is Not Exponential
July 21, 2015
Myth 6: At the End of PCR, Amplification Efficiency Is Not Exponential Because the Primers or NTPs Are Exhausted or the Polymerase Looses Activity PCR amplification occurs with a characteristic “S” shape. During the early cycles of PCR, the amplification... Read more ›
Why BLAST Search is a Myth for Determining the Specificity of a Primer
July 21, 2015
Myth 5: A BLAST Search Is the Best Method for Determining the Specificity of a Primer To minimize mispriming, several PCR texts suggest performing a BLAST search, and such capability is a part of some primer design packages such... Read more ›